The scope of the project

The scope of the project is to develop and test a novel and innovative optical fibre measurement system in order to investigate in real time the ethanol fermentation taking into acount the simultaneous measuring bioprocess parameters of interest (concentration of ethanol, glucose, lactic acid, CO2, temperature and pH) using an artificial neural network, with applications in all the fields of Bioeconomy: Agro-Food, Bioenergy (biogas, biomass, biofuel) and Biotechnology, in order to increase the economical and technological efficiency.

Sensors involving light measurements associated with fiber-optics are particularly attractive. The different configurations for fiber optic based sensors, their characteristics and their potential applications have been recently studied. Optical fibers can be used as sensors to measure temperature, pressure, pH, gases (oxigen, CO2) and other quantities by modifying a fiber so that the property to measure modulates the intensity, phase, polarization, wavelength, or transit time of light in the fiber. A major benefit of optical sensors is their ability to reach otherwise inaccessible places and can, if required, to provide distributed sensing over distances of up to one meter.

Recently, optical fiber was used as biochemical sensors. The fiber optic function is to transmit light to and from an immobilized reagent phase consisting generally of a fluorescent dye or of a colorimetric indicator secured at the end of the fiber optic. For biochemical analysis, substrates or enzymes can be immobilized, allowing the determination of enzyme activities or substrates. The enzyme immobilization procedure itself is one of the main factors that affect the performance of a biosensor.

Bragg gratings, written into optical fiber, have had an enormous impact since the late 1980s. Fiber Bragg Gratings (FBG) have moved from laboratory interest and curiosity to the brink of implementation in optical communication and sensor systems. The basic principle of operation of an FBG sensor system lies in the monitoring of the shift in wavelength of the returned “Bragg” signal, as a function of the measure entity. The principle behind the operation of FBG is in fact Fresnel reflection.

General objective

Developing and testing a novel optical fibre measurement system in order to investigate in real time the ethanol fermentation bioprocess. Result indicator: A novel valid real time optical fiber measurement system.

1. Fundamental research in order to develop and test the new optical sensors and the software for data acquisition and analysis

Output indicators: Optical sensor for glucose, Optical sensor for lactic acid, Optical sensor for ethanol, Specific software for data acquisition and analysis

2. Establishing the fundamental research procedure for measurements in real time using optical fiber sensors and the sensors validation

Output indicator: Validated optical sensors, Optical sensors system for ethanolic fermentation reaction

3. The elaboration of the artificial neuronal network and the optimisation of the real time monitoring system

Output indicators: Artificial neuronal network, Validated optical sensors system

Stages

STAGE A1 - Fundamental research on enzyme immobilization methods on optical fiber

A.1.1 - Theoretical and practical research regarding different methods for enzyme immobilisation onto optical fiber

Short description: Theoretical and practical research will be realised regarding enzyme immobilisation onto optical fiber using different methods (sol-gel matrix, entrapment, etc) This activity will be conducted starting with the 1st month- until the 3th month of the project

- Project members involved in this activity: Caraban A, Tarca R, Tarca I

- Deliverables: Study regarding different methods for enzyme immobilisation onto optical fiber

A.1.2 - Teoretical and practical research regarding FBG optical sensors for ethanol and glucose determination

Short description: Theoretical and practical research will be realised regarding the development of FBG sensors used for ethanol and glucose determination

- This activity will be conducted starting with the 1st month- until the 3th month of the project

- Project members involved in this activity: Caraban A, Tarca R, Tarca I

- Deliverables: Study regarding FBG optical sensors for ethanol and glucose determinations

STAGE A2 – Developing the experimental models of the optical enzymatic biosensors

A.2.1. Developing and testing the optical biosensors for glucose, ethanol and lactic acid

A.2.1.1.Developing the new types of optical sensor using different methods of enzyme immobilisation and FBG optical sensors

Short description: New types of optical sensor will be developed using different methods of enzyme immobilisation onto optical fiber and FBG optical sensors

- This activity will be conducted starting with the 4th month - until the 9th month of the project

- Project members involved in this activity: Caraban A, Bota S, Cozma A, Tarca R,Tarca I.

- Deliverables: Optical biosensor for glucose, Optical biosensor for ethanol, Optical biosensor for lactic acid

A.2.1.2.Testing the new optical sensors

Short description: The new types of optical sensor will be tested in pure systems and in ethanol fermentative reactions.

This activity will be conducted starting with the 6th month - until the 10th month of the project

- Project members involved in this activity: Caraban A, Bota S, Cozma A, Tarca R,Tarca I.

- Deliverables: Optical biosensor for glucose, Optical biosensor for ethanol, Optical biosensor for lactic acid

A.2.2. Developing the software for new optical sensors monitoring in real time

Short description: For the new optical sensors it will be developed the software for real time monitoring

- This activity will be conducted starting with the 7st month- until the 11th month of the project

- Project members involved in this activity:Noje D, Birouas, Tocut D, Moldovan O.

- Deliverables: Softwares for biosensors

STAGE A3 – Technological validation and lab optimization of the optical sensors system for the real-time monitoring of the fermentation bioprocess

A.3.1. Enzyme biosensors validation using spectrophotometer and HPLC methods

Short description: The new enzyme biosensor will be validated using classical spectrophotometer and HPLC methods

- This activity will be conducted starting with the 12th month- until the 13th month of the project

- Project members involved in this activity: Caraban A, Bota S, Cozma A

- Deliverables: Validated enzymatic biosensors

A.3.2. The optimisation of the real time monitoring ethanol fermentation biotechnological process using optical sensors system

A.3.2.1. Developing and testing the new optical sensors system for real time monitoring fermentation process

Short description: The new optical sensor system will be developed and tested using as components the new optical biosensors for glucose, ethanol and lactic acid together with the optical sensors for temperature, pH and CO2 available before the project.

This activity will be conducted starting with the 11st month- until the 18th month of the project

- Project members involved in this activity: Caraban A, Bota S, Cozma A, Tarca R,Tarca I.

- Deliverables: Optical sensors system for ethanol fermentation reaction

A.3.2.2.Developing and testing the Neuronal Network for real time monitoring fermentation process parameters

Short description: In order to optimisation of the real time monitoring ethanol fermentation biotechnological process using optical fibre sensors it will be developed a neuronal network to monitoring of the bioprocess evolution taking into account the real variation of all the considered parameters in their interdependence.

This activity will be conducted starting with the 8st month- until the 18th month of the project

- Project members involved in this activity: Vesseleny T, Noje D, Moldovan O, Birouas

A.3.3. Results interpretation, correlation and dissemination

Short description: This activity will be conducted starting with the 8th month- until the 18th month of the project

- Project members involved in this activity: Caraban A, Tarca R,Tarca I, Vesellenyi, Noje, Moldovan, Biroas

- Deliverables: request for patent, 3 ISI, 4 BDI

Budget

An Partner Value
2017 University of Oradea 176.470 RON
2017 Primus Technologies SRL 0 RON
2018 University of Oradea 226.015,69 RON
2018 Primus Technologies SRL 109.379,15 RON
Total 511.864,84 RON

Team

Person

Tarca Radu

expert in sensors systems, in DAQ systems – in particular for optical fiber sensor and competences in AI (neural network, fuzzy systems), project management

Person

Caraban Alina

expert in biochemistry, enzyme studies and kinetics, enzyme immobilisation, biotechnology, project leader HURO 1001/121 acronim Bioethanol

Person

Bota Sanda

chemistry expert in HPLC analysis, organic chemistry and biochemistry, enzyme immobilisation

Person

Cozma Alina

expert in analytical chemistry, HPLC analysis, spectrophotometric analysis, experimental data analysis

Person

Vesselenyi Tiberiu

expert in sensors systems, in DAQ systems and competences in AI (neural network, fuzzy systems, genetic algorithms)

Person

Tarca Ioan

expert in sensors systems, in programming, in DAQ systems and competences in AI (neural network, fuzzy systems)

Person

Moldovan Ovidiu

expert in sensors systems, in DAQ systems and competences in AI (neural network, fuzzy systems)

Person

Birouas Flavius

expert in programming, Visual C++, MikroC, MPLABX, LabView, CAT software, Linux Bash Shell Scripting

Person

Tocut Pavel Danut

expert in sensors and administrative tasks

Person

Noje Dan

software architecture expert, ISO27001:2013 certified auditor, fuzzy systems and MV Systems, project management

Results

ISI

1. IoT Devices Signals Processing with the Help of Multi-Dimensional Shepard Local Approximation Operators Defined in Riesz MV-Algebras, Noje Dan – Catalin, Tarca Radu, Dzitac Ioan, Pop Nicolae, INTERNATIONAL JOURNAL OF COMPUTERS COMMUNICATIONS & CONTROL, (1,29 IF), acceptat spre publicare in No.2/2019.

2 .Study About HPLC Method Elaboration and Validation for Acid Lactic Assay in Grape Juice Fermentation Process, Bota Sanda Rodica, Cozma Alina-Claudia, Cărăban Alina-Maria, Revista de Chimie, (1,41 IF), în evaluare.

3. The design and experimental development of air scanning using a sniffer quadcopter, Kuantama Endrowednes, Țarcă Ioan Constantin, Vesselenyi Tiberiu, Tarca Radu, Measurement, (2,218 IF), în evaluare.

4. IoT devices signals processing with the help of Shepard local approximation operators defined in Riesz MV-algebras, Noje Dan – Catalin, Dzitac Ioan, Pop Nicolae, Tarca Radu, Informatica (1,39 IF), în evaluare.

BDI

1. Data Aquisintion and Processing of Optical Fiber Bragg Grating Sensors, Birouaş Ionuț – Flaviu, Csokmai Lehel, Țarcă Dan Ioan, Tarca Radu, Annals of the University Of Oradea. Fascicle of Management and Technological Engineering, publicat;

2. A Multi-Parametric System ror Monitoring the Fermentation Process, Moldovan Ovidiu Gheorghe, Csokmai Lehel Szabolcs, Cărăban Alina-Maria, Tocut Pavel-Dănuț, Nonconventional Technologies Review, publicat;

3. Optimization the Method for Determination of Lactic Acid by HPLC Method, Bota Sanda Rodica, Cozma Alina-Claudia, Cărăban Alina-Maria, Natural Resources and Sustainable Development Journal, publicat;

4. Studies about the Alcohol Dehydrogenase Activity in Ethanol Fermentation using Optical Fibre, Cărăban Alina-Maria, Tarca Radu, Țarcă Ioan Constantin, Bota Sanda Rodica, Cozma Alina-Claudia, Țarcă Dan Ioan, Natural Resources and Sustainable Development Journal, publicat;

Conference

1. Lactat dehidrogenase immobilization using sol-gel method for application to enzymatic biosensor, Cărăban Alina-Maria, Bota Sanda Rodica, Moga Ioan, Conference NATURAL RESOURCES AND SUSTAINABLE DEVELOPMENT, 2018, prezentare orală.

Patent

1. Țarcă Radu, Sun Tong, Cărăban Alina, Țarcă Ioan Constantin, Kenneth Grattan, Senzor FBG pentru măsurarea indirectă a concentrației de etanol, - în curs de redactare.

Website

1. S-a realizat website-ul: https://imt.uoradea.ro/web/pniii_198ped_2017/. Website-ul a fost adus la zi în decembrie 2017 și decembrie 2018 pentru a include cele mai recente rezultate din proiect.

Perspective de colaborare

Universitatea din Oradea, prin coordonatorul acestui proiect, are o lungă colaborare cu colectivul condus de prof. Grattan Kenneth (Officer of the Order of the British Empire (OBE), fellow of Royal Academy of Engineering) de la City University of London. În urma și a acestui proiect au apărut noi perspective de colaborare cu colegii de la Londra. Astfel, prof. Grattan a făcut o vizită de trei zile în luna iunie la Oradea, prilej cu care s-au discutat posibilități de colaborare atât în domeniul măsurărilor utilizând senzori pe fibră optică, cât și în alte domenii.

Ca urmare a rezultatelor obtinute in cadrul proiectului, au fost deja demarate discutii cu Prof. Dieter Hertweck de la Reutlingen University din Germania, care este in stransa colaborare cu 2 centre de cercetare in vinificatie din Germania, dar si cu University of Maribor, universitate care are propria podgorie. Se doreste astfel dezvoltarea unui sistem automat dedicat monitorizarii procesului de vinificare.

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Contact

Universitatea din Oradea

Str. Universităţii nr. 1, Oradea, 410087, Bihor